Spatial-spectral coupling in coherent anti-Stokes Raman scattering microscopy

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DOIResolve DOI: http://doi.org/10.1364/OE.21.015298
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TypeArticle
Journal titleOptics Express
ISSN1094-4087
Volume21
Issue13
Pages1529815307; # of pages: 10
SubjectCoherent anti-Stokes Raman scattering microscopy; Focal positions; Hyperspectral Imaging; Retrieval algorithms; Retrieval methods; Spatial imaging; Spectral contribution; Third-order nonlinear optical; Raman scattering; Raman spectroscopy; Coherent scattering
AbstractCoherent anti-Stokes Raman scattering (CARS) microscopy is a third-order nonlinear optical technique which permits label-free, molecule-specific hyperspectral imaging. The interference between coherent resonant and non-resonant terms leads to well known distortions in the vibrational spectrum, requiring the use of retrieval algorithms. It also leads to spatial imaging distortions, largely due to the Gouy phase, when objects are smaller than the Rayleigh range. Here we consider that the focal position and spectral contributions to the nonlinear image formation are intrinsically coupled and cannot be corrected by conventional retrieval methods. © 2013 Optical Society of America.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC)
Peer reviewedYes
NPARC number21270643
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Record identifier009c08fd-aec6-4021-bfe8-60cf9b2c3bf0
Record created2014-02-17
Record modified2016-05-09
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