Cellodextrin utilization and β-glucosidase production by Bacteroides polypragmatus

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DOIResolve DOI: http://doi.org/10.1007/BF00413033
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TypeArticle
Journal titleArchives of Microbiology
Volume145
Pages9196; # of pages: 6
AbstractBacteroides polypragmatus, a mesophilic obligate anaerobe, was shown to simultaneously ferment glucose and cellobiose giving ethanol as a major metabolic end-product. A mixture of higher cellodextrins was also utilized. The bacterium produced a β-glucosidase with a pI value of 4.2 and a molecular weight of approximately 100000 daltons. The enzyme was intracellular and functioned optimally at pH 7. The K m values obtained with p-nitrophenyl-β-d-glucoside and cellobiose as substrates were 0.73 mM and 100 mM, respectively. The enzyme was quite stable at elevated temperatures; in the presence of 10% glycerol (v/v), it had a half-life of 4 h at 55°C. It was also stable during long-term storage at either 4°C or-20°C, provided that 10% (v/v) glycerol was added to preparations maintained at-20°C.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada
Peer reviewedNo
NRC numberMACKENZIE1986
25676
NPARC number9387828
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Record identifier01603cfa-0188-47f4-bf74-d9d916eabb13
Record created2009-07-10
Record modified2016-05-09
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