Effects of linear cationic a-helical antimicrobial peptides on immune-relevant genes in trout macrophages

Download
  1. Get@NRC: Effects of linear cationic a-helical antimicrobial peptides on immune-relevant genes in trout macrophages (Opens in a new window)
DOIResolve DOI: http://doi.org/10.1016/j.dci.2005.10.011
AuthorSearch for: ; Search for: ; Search for: ; Search for: ; Search for:
TypeArticle
Journal titleDevelopmental & Comparative Immunology
Volume30
Issue9
Pages797806; # of pages: 10
Subjectantimicrobial peptide; immune; trout; macrophage; IL-1; COX-2; RTS11; inflammation
AbstractThere are increasing evidence of the potential role of antimicrobial peptides in the regulation of immune responses in mammalian species. However, the effects of these peptides in fish have yet to be investigated. In this study, we examined the transcriptional expression profile of representative immune-relevant genes in a trout macrophage cell line, RTS11, in response to three linear cationic ?-helical antimicrobial peptides (insect cecropin B, fish pleurocidin and a cecropin analogue CF17). The expression levels of two pro-inflammatory genes, interleukin-1? (IL-1?) and cyclo-oxygenase-2 (COX-2), increased in the peptide-treated RTS11 cells. The peptides did not appear to affect the expression levels of representative genes associated with antigen presentation, interferon response or JAK/STAT signal transduction. Furthermore, the induction of IL-1? and COX-2 in RTS11 by lipopolysaccharide was not adversely affected by these three antimicrobial peptides. Overall, the data indicate a pro-inflammatory effect of the three cationic antimicrobial peptides in the inflammatory response of salmonid species, suggesting a potential application of these peptides as immune adjuvant for fish vaccination.
Publication date
PublisherElsevier
Copyright noticeCopyright © 2007 Elsevier B.V. All rights reserved.
LanguageEnglish
AffiliationNational Research Council Canada; NRC Institute for Marine Biosciences
Peer reviewedYes
NRC number1487
NPARC number3538499
Export citationExport as RIS
Report a correctionReport a correction
Record identifier026042b2-5ec2-4e88-9616-60f8be0b8b77
Record created2009-03-01
Record modified2016-05-09
Bookmark and share
  • Share this page with Facebook (Opens in a new window)
  • Share this page with Twitter (Opens in a new window)
  • Share this page with Google+ (Opens in a new window)
  • Share this page with Delicious (Opens in a new window)