Application of an immunoaffinity-based preconcentration method for mass spectrometric analysis of the O-chain polysaccharide of Aeromonas salmonicida from in vitro- and in vivo-grown cells

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  1. Get@NRC: Application of an immunoaffinity-based preconcentration method for mass spectrometric analysis of the O-chain polysaccharide of Aeromonas salmonicida from in vitro- and in vivo-grown cells (Opens in a new window)
DOIResolve DOI: http://doi.org/10.1111/j.1574-6968.2009.01612.x
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TypeArticle
Journal titleFEMS Microbiology Letters
Volume295
Issue2
Pages148155; # of pages: 8
SubjectAeromonas salmonicida; MS; lipopolysaccharide; identification
AbstractIn this study, application of magnetic beads (Dynabeads) coated with Aeromonas salmonicida lipopolysaccharide-specific polyclonal antisera to MS-based characterization of bacterial lipopolysaccharides has been evaluated. The results showed that the affinity-based preconcentration strategy resulted in at least a 100-fold increase in the detection of sensitivity, affording direct capillary electrophoresis (CE)-MS analysis of A. salmonicida lipopolysaccharide O-chain polysaccharide from in vitro-cultured cells. Subsequent CE-MS analysis of in vivo-grown cells of A. salmonicida confirmed significant changes in the structure of the lipopolysaccharide O-chain polysaccharide as a result of in vivo cultivation.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); NRC Institute for Biological Sciences; NRC Institute for Marine Biosciences
Peer reviewedYes
NPARC number15329306
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Record identifier033a462f-168e-40bb-b774-66d906c4a63d
Record created2010-05-21
Record modified2016-05-09
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