Optimization of triacylglycerol-estolide analysis by matrix-assissted laser desportion/ionization-mass spectrometry

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DOIResolve DOI: http://doi.org/10.1007/s11746-014-2437-y
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TypeArticle
Journal titleJournal of American Oil Chemists' Society
ISSN0003-021X
1558-9331
Volume91
Issue6
Pages905915; # of pages: 11
SubjectDHB; Ergot oil; Estolide; MALDI-MS; THAP; Triacylglycerol
AbstractAcylglycerols containing more than three acyl groups (TAG-estolides) have been reported in plant seed oils and oil from ergot fungus. These TAG-estolides have considerable potential for industrial use, however, costs of producing synthetic TAG-estolides limits their use in large-scale applications. Identification and structural characterization of additional natural sources of TAG-estolides has been restricted by their complexity and limitations of current analytical techniques. In this work, detection and characterization of TAG-estolides was optimized for use with MALDI-TOF-MS. Eight commonly used matrices were compared; 2,5-dihydroxybenzoic acid (DHB) and 2,4,6- trihydroxyacetophenone (THAP) gave good quality mass spectra. Matrix additives were examined and lithium was the most suitable, since MS/MS spectra of lithiated TAG-estolides provided the most informative fragmentation using an optimized method. The matrix solution pH was examined, and for THAP, replacing LiCl with 10-40 mM LiOH resulted in a slightly basic pH and significantly more intense TAG-estolide signals (up to eightfold higher). Since DHB is acidic, a larger amount of LiOH (>150 mM) was required for the matrix solution to become basic, leading to ion suppression and reduced signal intensities. Thus, for TAG-estolide analysis, THAP with ~20 to 30 mM LiOH gives the highest quality spectra and the most informative MS/MS fragmentation.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada; Aquatic and Crop Resource Development
Peer reviewedYes
NRC numberNRC-ACRD-56025
NPARC number21272403
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Record identifier04503c7d-02c9-43df-972a-583d81b9a394
Record created2014-11-25
Record modified2016-05-09
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