Geniposide protects pancreatic INS-1E β cells from hIAPP-induced cell damage: Potential involvement of insulin degrading-enzyme

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DOIResolve DOI: http://doi.org/10.1002/cbin.10394
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TypeArticle
Journal titleCell Biology International
ISSN1065-6995
Volume39
Issue4
Pages373378; # of pages: 6
Subjectgeniposide; insulinase; animal cell; cell damage; cell mediated cytotoxicity; cell protection; concentration response; controlled study; drug mechanism; enzyme activity; gene expression regulation; nonhuman; pancreas islet beta cell; protein aggregation; rat; upregulation
AbstractIslet amyloid deposition is increasingly seen as a pathogenic feature of type 2 diabetes mellitus (T2DM), with the deposits containing the unique amyloidogenic peptide islet amyloid polypeptide (IAPP, also known as amylin). The fibril precursors of IAPP contribute to its cytotoxicity on pancreatic β cells and be important in causing β-cell dysfunction in T2DM. However, the development of effective this study, inhibitors against the toxicity of IAPP has been extremely challenging. We have found that pre-incubation with geniposide dose-dependently prevented human IAPP (hIAPP)-induced cell damage in INS-1E cells, and bacitracin, an inhibitor of IDE activity, prevented significantly the protective effects of geniposide in pancreatic INS-1E cells significantly. Geniposide induced the expression of insulin-degrading enzyme (IDE), a key degrading protein of hIAPP, but had no significant effect on the aggregation of hIAPP. These findings indicate that geniposide prevents hIAPP-induced cytotoxicity in INS-1E cells involving upregulation of IDE expression.
Publication date
PublisherWiley
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); Aquatic and Crop Resource Development
Peer reviewedYes
NPARC number21275701
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Record identifier053f7359-9a1b-4221-b6e1-f052fa155b9c
Record created2015-07-14
Record modified2016-05-09
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