Characterization of interactions between LPS transport proteins of the Lpt system

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DOIResolve DOI: http://doi.org/10.1016/j.bbrc.2010.12.121
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TypeArticle
Journal titleBiochemical and Biophysical Research Communications
Volume404
Issue4
Pages10931098; # of pages: 6
SubjectLPS transport; Lpt system; LptC; LptA; LptE
AbstractThe lipopolysaccharide transport system (Lpt) in Gram-negative bacteria is responsible for transporting lipopolysaccharide (LPS) from the cytoplasmic surface of the inner membrane, where it is assembled, across the inner membrane, periplasm and outer membrane, to the surface where it is then inserted in the outer leaflet of the asymmetric lipid bilayer. The Lpt system consists of seven known LPS transport proteins (LptA-G) spanning from the cytoplasm to the cell surface. We have shown that the periplasmic component, LptA is able to form a stable complex with the inner membrane anchored LptC but does not interact with the outer membrane anchored LptE. This suggests that the LptC component of the LptBFGC complex may act as a dock for LptA, allowing it to bind LPS after it has been assembled at the inner membrane. That no interaction between LptA and LptE has been observed supports the theory that LptA binds LptD in the LptDE homodimeric complex at the outer membrane.
Publication date
LanguageEnglish
AffiliationNRC Biotechnology Research Institute; National Research Council Canada
Peer reviewedYes
NRC number53132
NPARC number16822447
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Record identifier06f0d0bb-bb3f-4deb-9e16-77b078e5b6e8
Record created2011-03-31
Record modified2016-05-09
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