A mechanobiological investigation of platelets

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DOIResolve DOI: http://doi.org/10.1007/s10237-010-0248-0
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Journal titleBiomechanics and Modeling in Mechanobiology
Pages473484; # of pages: 12
SubjectPlatelet; Human; Murine; Micropipette; Constitutive modeling
AbstractUnderstanding mechanotransduction pathways leading to thrombosis will require progressive steps, including determination of the mechanical behavior of the platelet membrane in response to applied loads. The platelet membrane deformation capacity, as quantified by membrane progression into a borosilicate glass micropipette of defined internal diameter, was probed in murine platelets using a controlled range of negative pressure (0–7cmH2O). Based on our observations that the platelet portion outside the micropipette was mostly spherical and that the platelet volume did not change upon aspiration, a novel continuum mechanics-based model of the platelet micropipette aspiration experiment was created, and a new hyperelastic isotropic material model including membrane residual tension was proposed for the platelet membrane. Murine platelet membranes maintained an average linear deformation behavior: Lp/Rp = 146,100pi × Rp + 19.923, where Lp is the platelet length aspirated in the micropipette (m), Rp is micropipette radius (m) and pi is the aspiration pressure (Pa). The theoretical model was used to generate material constants for the murine platelet membrane that allowed for an accurate simulation of the micropipette aspiration experiments. From published results, another set of material constants was established for the human platelet membrane. Limited cases of platelet lysis upon aspiration were analyzed using the theoretical model to determine preliminary membrane tension strength values.
Publication date
AffiliationNRC Institute for Biological Sciences; National Research Council Canada
Peer reviewedYes
NPARC number17620239
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Record identifier06ff6a06-4ff9-48ce-bf4e-7b6fcfbb0e6c
Record created2011-03-30
Record modified2016-05-09
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