Primary sequence and refined tertiary structure of Pseudomonas fluorescens Hole Azurin at 2.05 Å

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DOIResolve DOI: http://doi.org/10.1107/S0907444997001509
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TypeArticle
Journal titleActa Crystallographica Section D: Biological Crystallography
ISSN0907-4449
Volume53
Issue5
Pages493506; # of pages: 14
AbstractThis paper reports the primary sequence and refined crystal structure of Pseudomonas fluorescens holoazurin. The crystal structure has been determined by molecular replacement on the basis of the molecular model of azurin from Alcaligenes denitrificans, and refined by the method of molecular dynamics simulation and energy-restrained least-squares methods. P. fluorescens was crystallized in the orthorhombic space group P212121 with unit-cell dimensions a = 31.95, b = 43.78, c = 78.81 Å. The asymmetric unit is composed of only one molecule. The final R value is 16.7% for 6691 reflections to a resolution of 2.05 Å. This azurin structure shows some interesting features at His35 and His83. Part of the main chain of strand 3 including His35 O are involved in the contact between two symmetrically related molecules. P. fluorescens is also compared with the other azurin structures in terms of primary sequence, crystal packing, solvent structure and Cu-site geometry. The difference in fluorescence decay behavior of two holoazurins from P. fluorescens and P. aeruginosa and the correlation between the fluorescence quenching and electron transfer are discussed.
Publication date
LanguageEnglish
AffiliationNRC Institute for Biological Sciences; National Research Council Canada
Peer reviewedYes
NPARC number21275870
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Record identifier0736e934-8671-4b9b-9d93-af42828a6d31
Record created2015-07-20
Record modified2016-05-09
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