A Campylobacter jejuni glycosylation island important in cell charge, legionaminic acid biosynthesis and colonisation of chickens

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TypeArticle
Journal titleINFECTION AND IMMUNITY
SubjectACID; analysis; Animal; Biofilms; biosynthesis; Campylobacter; Campylobacter jejuni; Canada; cell; CHAIN; Chickens; DIFFERENCE; DISCOVERY; DISEASE; Flagellin; Form; GENE; Genes; Glycosylation; MECHANISMS; MOLECULAR; Mutagenesis; MUTANT; protein; Role; STRAIN; STRAINS; STRUCTURAL; structural analysis; STRUCTURAL-ANALYSIS; surface; veterinary
AbstractPreviously we identified five genes (Cj1321-26) in the O-linked flagellin glycosylation island that were highly prevalent in Campylobacter jejuni isolates from chickens. We report mutagenesis, functional and structural data to confirm that this locus, and Cj1324 in particular, has a significant contributory role in the colonisation of C. jejuni in chickens. A motile DeltaCj1324 mutant with intact flagellae was considerably less hydrophobic and less able to autoagglutinate and form biofilms than the parent strain 11168H, suggesting that the surface charge of flagellae was altered in Cj1324-deficient strains. These physical and functional attributes were restored upon complementation. Structural analysis of flagellin protein purified from the DeltaCj1324 mutant revealed the absence of two legionaminic acid glycan modifications that were present in the parent strain 11168H. These glycoform modifications were shown to be prevalent in chicken isolates and confirm that differences in the highly variable flagellin glycosylation locus can relate to strain source. The discovery of molecular mechanisms influencing persistence of C. jejuni in poultry aids the rational design of approaches to control this problematic pathogen in the food chain
Publication date
LanguageEnglish
AffiliationNational Research Council Canada; NRC Institute for Marine Biosciences; NRC Institute for Biological Sciences
Peer reviewedNo
NRC numberHOWARD2009
NPARC number9381698
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Record identifier07a525a1-6b0e-4e76-be88-a9e00df47636
Record created2009-07-10
Record modified2016-05-09
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