Ultrasound-mediated gene delivery into hard-to-transfect KG-1 cells

DOIResolve DOI: http://doi.org/10.1109/LISSA.2011.5754179
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TypeArticle
Proceedings title2011 IEEE/NIH Life Science Systems and Applications Workshop (LiSSA)
Conference2011 IEEE/NIH Life Science Systems and Applications Workshop, LiSSA 2011, April 7-8, 2011, Bethesda, MD, USA
ISBN9781457704208
978-1-4577-0421-5
Article number5754179
Pages143146; # of pages: 4
SubjectLipofectamine; Synergistic properties; Transfection reagents; Ultrasound-mediated gene delivery; Gene therapy; Gene transfer; Liposomes; Ultrasonics
AbstractIn this paper, we explore the possibility of increasing the transfection rate of hard-to-transfect cells (such as KG-1 cells) using ultrasound-mediated gene delivery (USD). Single application ultrasound induced as well as the synergistic properties of ultrasound with commonly used transfection reagents and repeated sessions USD were investigated and evaluated using GFP plasmid. KG-1 cells were not transfected by combining a commonly used transfection reagent and USD. However, repeated ultrasound-mediated treatments were able to increase transfection rate to 15% with a viability of 97%, as compared to 2% with the transfection reagent, Lipofectamine, alone. © 2011 IEEE.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); National Institute for Nanotechnology
Peer reviewedYes
NPARC number21271995
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Record identifier080008ea-cd95-4ba8-8fc9-aaa255be2200
Record created2014-05-16
Record modified2016-05-09
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