Development of a fast extraction method and optimization of liquid chromatography–mass spectrometry for the analysis of phenolic compounds in lentil seed coats

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DOIResolve DOI: http://doi.org/10.1016/j.jchromb.2014.08.007
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TypeArticle
Journal titleJournal of Chromatography B
ISSN1570-0232
Volume969
Pages149161; # of pages: 13
SubjectAcetone; Chromatography; Extraction; Flavonoids; High performance liquid chromatography; Mass spectrometry; Phenols; Solvents; Chromatographic conditions; Condition optimizations; Core-shell columns; Lens culinaris; Liquid chromatography - mass spectrometries; Liquid chromatography-Mass spectrometry; Organic compositions; Polyphenols; Drug isolation; Drug screening; Flow rate; Genotype; Lentil; Nonhuman; Plant breeding; Priority journal; Seed husk; Ultraviolet radiation
AbstractA systematic set of optimization experiments was conducted to design an efficient extraction and analysis protocol for screening six different sub-classes of phenolic compounds in the seed coat of various lentil (Lens culinaris Medik.) genotypes. Different compounds from anthocyanidins, flavan-3-ols, proanthocyanidins, flavanones, flavones, and flavonols sub-classes were first optimized for use as standards for liquid chromatography mass spectrometry (LC-MS) with UV detection. The effect of maceration duration, reconstitution solvent, and extraction solvent were investigated using lentil genotype CDC Maxim. Chromatographic conditions were optimized by examining column separation efficiencies, organic composition, and solvent gradient. The results showed that a 1. h maceration step was sufficient and that non-acidified solvents were more appropriate; a 70:30 acetone: water (v/v) solvent was ultimately selected. Using a Kinetex PFP column, the organic concentration, gradient, and flow rate were optimized to maximize the resolution of phenolic compounds in a short 30-min analysis time. The optimized method was applied to three lentil genotypes with different phenolic compound profiles to provide information of value to breeding programs.
Publication date
LanguageEnglish
AffiliationAquatic and Crop Resource Development; National Research Council Canada
Peer reviewedYes
NRC numberNRC-ACRD-56082
NPARC number21273872
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Record identifier0b319d3c-ec03-47dc-b84d-0662d6b9db5b
Record created2015-01-30
Record modified2016-05-09
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