Metabolic interactions between the Lands Cycle and the Kennedy Pathway of Glycerolipid synthesis in Arabidopsis developing seeds

Alternative titleMetabolic interactions between the Lands Cycle and the Kennedy Pathway of Plant Glycerlipid synthesis
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DOIResolve DOI: http://doi.org/10.1105/tpc.112.104604
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TypeArticle
Journal titleThe Plant Cell
ISSN1040-4651
1532-298X
Volume24
Issue11
Pages46524669; # of pages: 18
AbstractIt has been widely accepted that the primary function of the Lands cycle is to provide a route for acyl remodeling to modify fatty acid (FA) composition of phospholipids derived from the Kennedy pathway. Lysophosphatidylcholine acyltransferase (LPCAT) is an evolutionarily conserved key enzyme in the Lands cycle. In this study, we provide direct evidence that the Arabidopsis thaliana LPCATs, LPCAT1 and LPCAT2, participate in the Lands cycle in developing seeds. In spite of a substantially reduced initial rate of nascent FA incorporation into phosphatidylcholine (PC), the PC level in the double mutant lpcat1 lpcat2-2 remained unchanged. LPCAT deficiency triggered a compensatory response of de novo PC synthesis and a concomitant acceleration of PC turnover that were attributable at least in part to PC deacylation. Acyl-CoA profile analysis revealed complicated metabolic alterations rather than merely reduced acyl group shuffling from PC in the mutant. Shifts in FA stereospecific distribution in triacylglycerol of the mutant seed suggested a preferential retention of saturated acyl chains at the stereospecific numbering (sn)-1 position from PC and likely a channeling of lysophosphatidic acid, derived from PC, into the Kennedy pathway. Our study thus illustrates an intricate relationship between the Lands cycle and the Kennedy pathway.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada; Aquatic and Crop Resource Development; NRC Plant Biotechnology Institute
Peer reviewedYes
NRC number54658
NPARC number21268461
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Record identifier0b793346-d947-47c9-998d-ec5857192b44
Record created2013-07-24
Record modified2016-05-09
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