The characterization of the O-antigen of Escherichia coli 064:K99 lipopolysaccharide: Carbohydr.Res.

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TypeArticle
Journal titleCarbohydr.Res.
Volume248
Pages277284; # of pages: 8
Subject1D; 2D; Acetylation; ACID; ANTIGEN; Antigens; bacterial; Canada; Carbohydrate Conformation; Carbohydrate Sequence; chemistry; Chromatography,Gel; DEGRADATION; Escherichia; Escherichia coli; ESCHERICHIA-COLI; growth & development; Hydrolysis; immunology; isolation & purification; LIPOPOLYSACCHARIDE; Lipopolysaccharides; LPS; MAGNETIC; Magnetic Resonance Spectroscopy; MAGNETIC-RESONANCE; Methylation; MOLECULAR; Molecular Sequence Data; Molecular Weight; NUCLEAR; Nuclear Magnetic Resonance; NUCLEAR-MAGNETIC-RESONANCE; O antigen; O Antigens; O-antigen; O-chain; oligosaccharide; Oligosaccharides; POLYSACCHARIDE; Polysaccharides; Polysaccharides,Bacterial; REPEATING UNIT; RESONANCE; SMOOTH; structure; UNIT
AbstractThe structure of the O-polysaccharide of the smooth lipopolysaccharide (LPS) produced by Escherichia coli O64:K99 was investigated by SDS-PAGE, composition, periodate oxidation, methylation, partial hydrolysis, and 1D and 2D nuclear magnetic resonance analyses, made on the native o-chain and its reduction and periodate degradation products. The E. coli O64 antigenic O-chain was found to be a high molecular weight glycan composed of D-galactose, D-glucuronic acid, 2-acetamido-2-deoxy-D-glucose, and 2-acetamido-2-deoxy-D-mannose (2:1:1:1) and was a polymer of branched pentasaccharide repeating units having the structure: [formula: see text]
Publication date
LanguageEnglish
AffiliationNRC Institute for Biological Sciences; National Research Council Canada
Peer reviewedNo
NRC numberPERRY1993
NPARC number9375788
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Record identifier0c7a6751-947f-4b9c-bcba-fa8eeb3dba0d
Record created2009-07-10
Record modified2016-05-09
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