Hydrophilic interaction liquid chromatography/mass spectrometry for determination of domoic acid in Adriatic shellfish

Download
  1. Get@NRC: Hydrophilic interaction liquid chromatography/mass spectrometry for determination of domoic acid in Adriatic shellfish (Opens in a new window)
DOIResolve DOI: http://doi.org/10.1002/rcm.2021
AuthorSearch for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for:
TypeArticle
Journal titleRapid Communications in Mass Spectrometry
Volume19
Issue14
Pages20302038; # of pages: 9
Subjectliquid chromatography; mass spectrometry; Adriatic shellfish; domoic acid
AbstractThis paper describes a new method for sensitive, specific and direct determination of domoic acid (DA), the causative toxin of amnesic shellfish poisoning (ASP) syndrome, in shellfish. It is based on combination of hydrophilic interaction liquid chromatography with mass spectrometry (HILIC/MS). The high percentage of organic modifier in the mobile phase and the omission of ion-pairing reagents, both favoured in HILIC, result in enhanced detection limits with MS detection. The new method was set up either on an ionspray ion trap MS instrument operating in MS and MS/MS scanning acquisition modes, or on a turboionspray triple-quadrupole MS system operating in selected ion monitoring (SIM) and multiple reaction monitoring (MRM) acquisition modes. Positive and negative ion experiments were performed. MRM experiments are recommended for screening contaminated shellfish tissue and for quantitative analyses due to highest sensitivity and selectivity. The minimum detection levels for the toxin in tissue were found to be 63 and 190 ng/g in positive and negative MRM experiments, respectively, which are well below the regulatory limit for DA in tissue (20 µg/g). Application to shellfish samples collected in the Adriatic Sea (Italy) in the period 2000-2004 demonstrated for the first time in Italy the presence of DA as a new toxin that has entered the Adriatic Mytilus galloprovincialis toxin profile.
Publication date
PublisherWiley
Copyright noticeCopyright © 2005 John Wiley & Sons, Ltd.
LanguageEnglish
AffiliationNRC Institute for Marine Biosciences; National Research Council Canada; Measurement Science and Standards
Peer reviewedYes
NRC number1659
NPARC number3538105
Export citationExport as RIS
Report a correctionReport a correction
Record identifier0c8eff88-e4fd-4a44-9d50-60686477843e
Record created2009-03-01
Record modified2016-05-09
Bookmark and share
  • Share this page with Facebook (Opens in a new window)
  • Share this page with Twitter (Opens in a new window)
  • Share this page with Google+ (Opens in a new window)
  • Share this page with Delicious (Opens in a new window)