Localization of DIR1 at the tissue, cellular and subcellular levels during Systemic Acquired Resistance in Arabidopsis using DIR1:GUS and DIR1:EGFP reporters

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DOIResolve DOI: http://doi.org/10.1186/1471-2229-11-125
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TypeArticle
Journal titleBMC Plant Biology
ISSN1471-2229
Volume11
Article number125
SubjectLycopersicon esculentum; Nicotiana tabacum; Pseudomonas syringae; Arabidopsis protein; carrier protein; dir1 1 protein, Arabidopsis; plant RNA; cell wall; disease resistance; metabolism; pathogenicity; plant immunity; plant leaf; promoter region; Pseudomonas syringae; reporter gene; seedling; tobacco; transgenic plant; Gene Expression Regulation, Plant; Plants, Genetically Modified
AbstractBackground: Systemic Acquired Resistance (SAR) is an induced resistance response to pathogens, characterized by the translocation of a long-distance signal from induced leaves to distant tissues to prime them for increased resistance to future infection. DEFECTIVE in INDUCED RESISTANCE 1 (DIR1) has been hypothesized to chaperone a small signaling molecule to distant tissues during SAR in Arabidopsis.Results: DIR1 promoter:DIR1-GUS/dir1-1 lines were constructed to examine DIR1 expression. DIR1 is expressed in seedlings, flowers and ubiquitously in untreated or mock-inoculated mature leaf cells, including phloem sieve elements and companion cells. Inoculation of leaves with SAR-inducing avirulent or virulent Pseudomonas syringae pv tomato (Pst) resulted in Type III Secretion System-dependent suppression of DIR1 expression in leaf cells. Transient expression of fluorescent fusion proteins in tobacco and intercellular washing fluid experiments indicated that DIR1's ER signal sequence targets it for secretion to the cell wall. However, DIR1 expressed without a signal sequence rescued the dir1-1 SAR defect, suggesting that a cytosolic pool of DIR1 is important for the SAR response.Conclusions: Although expression of DIR1 decreases during SAR induction, the protein localizes to all living cell types of the vasculature, including companion cells and sieve elements, and therefore DIR1 is well situated to participate in long-distance signaling during SAR. © 2011 Champigny et al; licensee BioMed Central Ltd.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); NRC Genomics and Health Initiative; Aquatic and Crop Resource Development
Peer reviewedYes
NPARC number21271977
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Record identifier0d94f339-449f-4cb6-9aa5-a28eda1d29ac
Record created2014-05-15
Record modified2016-05-09
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