Iodination on tyrosine residues during oxidation with sodium periodate in solid phase extraction of N-linked glycopeptides

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DOIResolve DOI: http://doi.org/10.1007/s13361-011-0262-1
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TypeArticle
Journal titleJournal of The American Society for Mass Spectrometry
ISSN1044-0305
1879-1123
Volume23
Issue1
Pages6875; # of pages: 8
SubjectGlycoproteomics; Iodination; N-linked glycoproteins; Hydrazide modified solid phase extraction; Oxidation; Sodium periodate
AbstractSolid-phase extraction of N-linked glycopeptides (SPEG) using hydrazide-modified supports has become a common sample preparation procedure in glycoproteomic experiments. We demonstrate that iodination of tyrosine residues occur in SPEG as a side reaction during an oxidation step with sodium periodate. MS/MS analysis of oxidized bovine serum albumin and carbonic anhydrase digests revealed a characteristic shift of m/z 125.9 on all y and b fragment ions containing the modified tyrosine residues. Selected reaction monitoring (SRM) measurements showed that the peak intensity from of the iodinated peptides increased during the course of oxidation. After an hour of oxidation, SRM analysis revealed that the strongest signal from an iodinated peptide was approximately one-tenth of the intensity of the corresponding unmodified peptide. Iodinated tyrosine residues were also identified in serum samples subjected to SPEG and analyzed by LC-ESI-MS/MS. We recommend assessing this side reaction by including iodotyrosine as a variable modification when performing database searches on SPEG experiments. For SRM-based acquisitions, we encourage the avoidance of tyrosine-containing glycopeptides or, if this is not practical, monitoring transitions that contain the potential modified iodinated tyrosine residue to monitor the presence of the iodinated form of the glycopeptide.
Publication date
LanguageEnglish
AffiliationNRC Institute for Marine Biosciences; National Research Council Canada
Peer reviewedYes
Identifier262
NPARC number21268626
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Record identifier0dcf4b3e-f490-4c73-9cfc-b3438a967f67
Record created2013-10-31
Record modified2016-05-09
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