Kinetics of CO conversion into H2 by Carboxydothermus hydrogenoformans

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Journal titleApplied Microbiology and Biotechnology
Pages16771684; # of pages: 8
AbstractThe objective of this study was to improve the biological water-gas shift reaction for producing hydrogen (H2) by conversion of carbon monoxide (CO) using an anaerobic thermophilic pure strain, Carboxydothermus hydrogenoformans. Specific hydrogen production rates and yields were investigated at initial biomass densities varying from 5 to 20 mg volatile suspended solid (VSS) L-1. Results showed that the gas-liquid mass transfer limits the CO conversion rate at high biomass concentrations. At 100-rpm agitation and at CO partial pressure of 1 atm, the optimal substrate/biomass ratio must exceed 5 mol CO g-1 biomass VSS in order to avoid gas-liquid substrate transfer limitation. An average H2 yield of 94±3% and a specific hydrogen production rate of ca. 3 mol g-1 VSS day-1 were obtained at initial biomass densities between 5 and 8 mg VSS-1. In addition, CO bioconversion kinetics was assessed at CO partial pressure from 0.16 to 2 atm, corresponding to a dissolved CO concentration at 70°C from 0.09 to 1.1 mM. Specific bioactivity was maximal at 3.5 mol CO g-1 VSS day-1 for a dissolved CO concentration of 0.55 mM in the culture. This optimal concentration is higher than with most other hydrogenogenic carboxydotrophic species. © 2011 Her Majesty the Queen in Right of Canada.
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AffiliationNRC Biotechnology Research Institute; National Research Council Canada
Peer reviewedYes
NRC number53371
NPARC number18702532
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Record identifier0e6e5f70-b493-4c56-b8d7-8a0147504325
Record created2012-03-06
Record modified2016-05-09
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