Myxinidin, a Novel Antimicrobial Peptide from the Epidermal Mucus of Hagfish, Myxine glutinosa L.

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DOIResolve DOI: http://doi.org/10.1007/s10126-009-9189-y
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TypeArticle
Journal titleMarine Biotechnology
ISSN1436-2228
1436-2236
Volume11
Issue6
Pages748757; # of pages: 10
SubjectMucus; Antimicrobial peptide; Innate immunity; Hagfish; Myxinidin
AbstractFish epidermal mucus contains innate immune components that provide a first line of defense against various infectious pathogens. This study reports the bioassay-guided fractionation and characterization of a novel antimicrobial peptide, myxinidin, from the acidic epidermal mucus extract of hagfish (Myxine glutinosa L.). Edman sequencing and mass spectrometry revealed that myxinidin consists of 12 amino acids and has a molecular mass of 1,327.68 Da. Myxinidin showed activity against a broad range of bacteria and yeast pathogens at minimum bactericidal concentration (MBC) ranging from 1.0 to 10.0 µg/mL. Screened pathogens, Salmonella enterica serovar Typhimurium C610, Escherichia coli D31, Aeromonas salmonicida A449, Yersinia ruckeri 96-4, and Listonella anguillarum 02-11 were found to be highly sensitive to myxinidin at the MBC of 1.0–2.5 µg/mL; Staphylococcus epidermis C621 and yeast (Candida albicans C627) had an MBC of 10.0 µg/mL. The antimicrobial activity of myxinidin was found to be two to 16 times more active than a potent fish-derived antimicrobial peptide, pleurocidin (NRC-17), against most of the screened pathogens. The microbicidal activity of myxinidin was retained in the presence of sodium chloride (NaCl) at concentrations up to 0.3 M and had no hemolytic activity against mammalian red blood cells. These results suggest that myxinidin may have potential applications in fish and human therapeutics.
Publication date
PublisherSpringer
LanguageEnglish
AffiliationNational Research Council Canada; NRC Institute for Marine Biosciences; NRC Industrial Research Assistance Program
Peer reviewedYes
NRC number55469
NPARC number21268309
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Record identifier0e9acbb8-2911-4c82-af5e-21a1e073dcf0
Record created2013-06-19
Record modified2016-05-09
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