Effective control of yellow stem borer and rice leaf folder in transgenic rice indica varieties Basmati 370 and M 7 using the novel δ-endotoxin cry2A Bacillus thuringiensis gene

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DOIResolve DOI: http://doi.org/10.1023/A:1009660315970
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TypeArticle
Journal titleMolecular Breeding
Volume4
Pages501507; # of pages: 7
SubjectBacillus thuringiensis (Bt; cry2A; particle bombardment; rice leaf folder; transgenic rice; yellow stem borer;
AbstractTransgenic rice indica varieties Basmati 370 and M 7 expressing the novel cry2A (Bt) insecticidal gene were generated by particle bombardment. Molecular and biochemical analyses in R0 and R1 populations confirmed stable integration and expression of this novel Bt transgene. We estimated that the gene product was expressed up to 5% of total leaf protein. Insect feeding bioassays demonstrated that the Cry2A protein was effective against the yellow stem borer and the rice leaf folder, two major rice pests in the Indian Subcontinent. This is the first report of the control of major rice pests using this specific Bt gene. The cry2A gene can now be used in combination with other insecticidal genes for pyramiding resistance against insect pests. This will delay, or perhaps in combination with integrated pest management practices, prevent evolution of insect populations resistant to single insecticidal genes.
Publication date
LanguageEnglish
AffiliationNRC Biotechnology Research Institute; National Research Council Canada
Peer reviewedNo
NRC number41844
NPARC number3538689
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Record identifier0f3799e3-0d82-4fa7-b9f2-6fe4afb5147a
Record created2009-03-01
Record modified2016-05-09
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