Structural analysis of the lipooligosaccharide-derived oligosaccharide of Histophilus somni (Haemophilus somnus) strain 8025: Carbohydr.Res.

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TypeArticle
Journal titleCarbohydr.Res.
Volume341
Issue2
Pages281284; # of pages: 4
Subjectanalysis; biosynthesis; Canada; core oligosaccharide; Glycosyltransferases; Haemophilus; IDENTIFICATION; Laboratories; LACTOSE; lipooligosaccharide; mass spectrometry; MASS-SPECTROMETRY; method; Methods; Methylation; NMR; NMR spectroscopy; NMR-spectroscopy; oligosaccharide; Role; SPECTROSCOPY; STRAIN; STRAINS; STRUCTURAL; structural analysis; STRUCTURAL-ANALYSIS; structure
AbstractPrevious structural studies in our laboratory on lipooligosaccharide (LOS) inner core oligosaccharide (OS) had identified structures from several strains of Histophilus (Haemophilus) somni (738, 2336, 1P, 129Pt). Recently a type strain 8025 was proposed for this species and we therefore sought to determine the core OS structure of this H. somni strain. Core OS was isolated by standard methods from Westphal purified LOS. Structural information was established by a combination of monosaccharide and methylation analyses, NMR spectroscopy and mass spectrometry. The following structure for the core OS was determined on the basis of the combined data from these experiments: [carbohydrates: see text]. The structure determined contains aspects of other Histophilus somni core OS structures, such as the beta-Gal attached at the 2-position of Hep II (2336), PEtn only at the 6-position of Hep II (738, 129Pt) and a lactose extension from Hep I (1P). Since genetic manipulation has been achieved with this strain, the identification of the core OS structure will enable experiments designed to identify the role of glycosyltransferases involved in LOS biosynthesis
Publication date
LanguageEnglish
AffiliationNRC Institute for Biological Sciences; National Research Council Canada
Peer reviewedNo
NRC numberSTMICHAEL2006
NPARC number9377819
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Record identifier0f911d14-adfc-4ce1-8a00-d42913b89e25
Record created2009-07-10
Record modified2016-05-09
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