Characterization of cryoinjury-induced infarction with manganese-and-gadolinium-enhanced MRI and optical spectroscopy in pig hearts

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DOIResolve DOI: http://doi.org/10.1016/j.mri.2010.02.001
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TypeArticle
Journal titleMagnetic Resonance Imaging
Volume28
Issue5
Pages753766; # of pages: 14
SubjectAcute and chronic cryoinjury; No-reflow embolization; Gd-enhanced MRI; Mn-enhanced MRI; Optical imaging and spectroscopy
AbstractPurpose: To investigate progression of cryoinjury in pigs using contrast-enhanced magnetic resonance imaging (MRI) as well as optical spectroscopy and imaging. Methods: Cryoinjury was produced in 16 pigs in vivo and investigated using Gd-and Mn-enhanced MRI, optical imaging /spectroscopy and histology in acute and chronic setting up to 4 weeks after the injury. Results: (1) Acute cryoinjury resulted in formation of a lesion with a severely reduced rate of sub-epicardial indocyanine green (intravascular optical flow tracer) passage. In vivo late Gd-enhanced MRI showed a ?10 mm deep hypointense area that was surrounded by a hyperintense rim while ex vivo Mn-enhanced MRI (MEMRI) detected a homogenous hypointense zone. Histological and spectroscopic examination revealed embolic erythrocytes blockages within the cryolesion with a thin necrotic rim neighboring the normal myocardium. (2) Chronic 4-week cryoinjury was characterized by uniform Gd-enhancement, whereas MEMRI revealed reduced Mn2+enhancement. Histological examination showed replacement of the cryoinjured myocardium by scar tissue. Conclusions: Acute cryoinjury resulted in formation of a no-reflow core embolized by erythrocytes and surrounded by a rim of necrotic tissue. Upon injury progression, the no-reflow zone shrunk and was completely replaced with scar tissue by 4 weeks after injury.
Publication date
LanguageEnglish
AffiliationNRC Institute for Biodiagnostics; National Research Council Canada
Peer reviewedYes
NPARC number17653076
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Record identifier0f9a8683-e9e3-445b-a66d-c6172979949d
Record created2011-04-03
Record modified2016-05-09
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