Optimally chirped multimodal CARS microscopy based on a single Ti:sapphire oscillator

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DOIResolve DOI: http://doi.org/10.1364/OE.17.002984
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TypeArticle
Journal titleOptics Express
Volume17
Issue4
Pages29842996; # of pages: 13
SubjectCARS; microscopy; live cells; femtosecond; photonic crystal fiber; Spectral chirp; laser pulses; multimodal
AbstractWe demonstrate high performance coherent anti-Stokes Raman scattering (CARS) microscopy of live cells and tissues with user-variable spectral resolution and broad Raman tunability (2500 - 4100 cm-1), using a femtosecond Ti:Sapphire pump and photonic crystal fiber output for the broadband synchronized Stokes pulse. Spectral chirp of the fs laser pulses was a user-variable parameter for optimization in a spectral focusing implementation of multimodal CARS microscopy. High signal-to-noise, high contrast multimodal imaging of live cells and tissues was achieved with pixel dwell times of 2-8 µs and low laser powers (< 30 mW total).
Publication date
LanguageEnglish
AffiliationNational Research Council Canada; NRC Steacie Institute for Molecular Sciences
Peer reviewedYes
NPARC number16488518
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Record identifier10888da2-65c4-452b-bf25-c6627525f950
Record created2011-02-15
Record modified2016-05-09
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