Characterization of the antigenic O-polysaccharide produced by Escherichia coli serotype O:70

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DOIResolve DOI: http://doi.org/10.1016/j.carres.2009.11.026
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TypeArticle
Journal titleCarbohydrate Research
Volume345
Issue5
Pages644648; # of pages: 5
SubjectLipopolysaccharide; Escherichia coli O:70; Polysaccharide; Antigen
AbstractThe structure of the antigenic O-polysaccharide (O-PS) produced by Escherichia coli serotype O:70 was determined by analysis of the chromatographically purified O-PS polymer prepared by mild hydrolysis of its aqueous phenol-extracted smooth-type somatic lipopolysaccharide. The O-PS is composed of d-glucose, d-galactose, d-fucose, 2-acetamido-2-deoxy-d-galactose, and 3-acetamido-3-deoxy-d-quinovose in a ratio of 1:1:1:1:1. From the use of DOC–PAGE, methylation, Smith-type periodate oxidation, and ¹H and ¹³C NMR spectroscopy, including 2D experiments, the O-PS was shown to be a polymer of a branched repeating pentasaccharide unit having the structure
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LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); NRC Institute for Biological Sciences
Peer reviewedYes
NPARC number15335895
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Record identifier10e49bf4-3eff-4a43-8284-d8c3cf32205f
Record created2010-05-21
Record modified2016-05-09
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