Genotoxicity of 2,4- and 2,6-dinitrotoluene as measured by the Tradescantia micronucleus (Trad-MCB) bioassay

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DOIResolve DOI: http://doi.org/10.1016/S1383-5718(03)00089-5
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TypeArticle
Journal titleGenetic Toxicology and Environmental Mutagenesis
Volume538
Issue1-2
Pages1318; # of pages: 6
Subjectenv
AbstractThe phytogenotoxicity of 2,4-dinitrotoluene (2,4-DNT) and 2,6-dinitrotoluene (2,6-DNT) was assessed using the Tradescantia micronucleus (Trad-MCN) bioassay. Tradescantia cuttings bearing young inflorescences were exposed for 6 h to 2,4- or 2,6-DNT amended water solutions up to their respective solubilities. The nominal concentrations were 0, 1.9, 3.8, 7.5, 15, 30, 60, 100, 150, 200 mg/l of 2,4-DNT, and 0, 7.5, 15, 30, 60, 90, 120, 180 mg/l of 2,6-DNT. Each treatment was repeated three or four times. Chemical concentrations in test solutions were analyzed prior to and after the exposure. Cadmium chloride (0-20 mM) was used as the positive control. Micronuclei (MCN) were scored in the tetrad-stage pollen mother cells. The MCN frequency (%), i.e. the number of micronuclei scored in 100 tetrads, was the measurement endpoint. Results indicated that both 2,4-DNT and 2,6-DNT were genotoxic with the minimum effective dose (MED) of 30 and 135 mg/l, respectively. Longer exposure (30 h) without recovery time at 150 mg/l of 2,4-DNT and 180 mg/l of 2,6-DNT did not induce significantly higher MCN frequencies.
Publication date
AffiliationNRC Biotechnology Research Institute; National Research Council Canada
Peer reviewedNo
NRC number45914
NPARC number3540161
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Record identifier1137d791-61a8-4c58-b85c-29ac2b813774
Record created2009-03-01
Record modified2016-05-09
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