Real-time monitoring of influenza virus production kinetics in HEK293 cell cultures

  1. Get@NRC: Real-time monitoring of influenza virus production kinetics in HEK293 cell cultures (Opens in a new window)
DOIResolve DOI:
AuthorSearch for: ; Search for:
Journal titleBiotechnology Progress
Pages275284; # of pages: 10
Subjectinfluenza; cell culture; in-process monitoring; kinetics; vaccines
AbstractThere is an increased interest from the vaccine industry to use mammalian cell cultures for influenza vaccine manufacturing. Therefore, it became important to study the influenza infection mechanism, the viral–host interaction, and the replication kinetics from a bioprocessing stand point to maximize the influenza viral production yield in cell culture. In the present work, influenza replication kinetics was studied in HEK293 cells. Two infection conditions were evaluated, a low (0.01) and a high multiplicity of infection (1.0). Critical time points of the viral production cycle (infection, protein synthesis, viral assembly and budding, viral release, and host-cell death) were identified in small-scale cell cultures. Additionally, cell growth, viability, and viral titers were monitored in the viral production process. The infection state of the cultivated cell population was assessed by influenza immunolabeling throughout the culture period. Influenza virus production kinetics were also on-line monitored by dielectric spectroscopy and successfully correlated to real-time capacitance measures. Overall, this work provided insights into the mechanisms associated with the infection of human HEK293 cell line by the influenza virus and demonstrated, once again, the usefulness of multifrequency scanning permittivity for in-line monitoring and supervision of cell-based viral production processes.
Publication date
AffiliationHuman Health Therapeutics; National Research Council Canada
Peer reviewedYes
NRC number53191
NPARC number21268249
Export citationExport as RIS
Report a correctionReport a correction
Record identifier119f0649-07fc-4898-bb5d-44af3fbf34e8
Record created2013-06-10
Record modified2016-05-09
Bookmark and share
  • Share this page with Facebook (Opens in a new window)
  • Share this page with Twitter (Opens in a new window)
  • Share this page with Google+ (Opens in a new window)
  • Share this page with Delicious (Opens in a new window)