Exploring the diversity of marine-derived fungal polyketide synthases

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DOIResolve DOI: http://doi.org/10.1139/W06-131
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TypeArticle
Journal titleCanadian Journal of Microbiology
Volume53
Issue2
Pages291302; # of pages: 12
SubjectPKS; pigment; secondary metabolism; marine; fungi
AbstractUsing an approach based on polymerase chain reaction (PCR), we examined the diversity of polyketide synthase (PKS) genes present in 160 marine fungal isolates, representing 142 species. We obtained ketosynthase (KS) domain PCR products from 99 fungal isolates, representing Dothideomycetes, Sordariomycetes, Eurotiomycetes, and incertae sedis. Sequence similarity searches and phylogenetic analysis of 29 marine partial-KS-encoding sequences revealed domains predicted to encode reducing, nonreducing, and 6-methylsalicylic acid PKSs. Bioinformatic analysis of an alignment of the KS sequences from marine-derived fungi revealed no unique motifs in this region. However, several specificity-determining positions were apparent between fungal 6-methylsalicylic acid PKSs as compared with either reducing or nonreducing PKSs. Evaluation of these positions in the context of a modelled three-dimensional protein structure highlighted their potential use as PKS classification markers. Evaluating primer-binding sites was necessary to obtain KS domain fragments from putative PKSs while maintaining a level of sequence information adequate to properly classify and characterize them.
Publication date
LanguageFrench
AffiliationNational Research Council Canada; NRC Institute for Marine Biosciences
Peer reviewedYes
IdentifierNRC-ACRD-55930
NRC number1569
NPARC number3538434
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Record identifier1252aa80-40a3-4502-bc70-f9bd866ab8bf
Record created2009-03-01
Record modified2016-05-09
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