Immunocytochemical localization of pleurocidin to the cytoplasmic granules of eosinophilic granular cells from the winter flounder gill

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DOIResolve DOI: http://doi.org/10.1111/j.1095-8649.2007.01452.x
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TypeArticle
Journal titleJournal of Fish Biology
Volume70
IssueSupplement sc
Pages336345; # of pages: 10
SubjectEGC; gill; granules; immunocytochemistry; pleurocidin; winter flounder
AbstractThe teleost gill is considered to be of significant immunological importance, as it is one of the first tissues exposed to environmental or pathogenic challenge and thus should be well equipped to mount an effective immune response. This study characterizes ultrastructurally and immunocytochemically a tissue granulocyte (eosinophilic granular cell) from the winter flounder gill that was previously determined to be involved in the gene expression and synthesis of a known antimicrobial peptide (pleurocidin). The cell is irregular in shape with a cytoplasm characterized by numerous large, electron-dense, membrane-bounded granules. The nucleus is euchromatic and closely associated with a prominent rough endoplasmic reticulum. The cytoplasm typically contains two to three mitochondria and a centralized Golgi apparatus surrounded by numerous electron-lucent vesicles. Immunogold staining of the cells with an antipleurocidin antibody shows large number of gold particles in direct association with the electron-dense granules. These data provide the first evidence definitively showing storage of an antimicrobial peptide in the cytoplasmic granules of an eosinophilic granule cell resident in gill tissue.
Publication date
PublisherWiley
Copyright notice2007 Crown copyright
Journal compilation Copyright 2007 The Fisheries Society of the British Isles
LanguageEnglish
AffiliationNational Research Council Canada; NRC Institute for Marine Biosciences; Aquatic and Crop Resource Development
Peer reviewedYes
IdentifierNRC-ACRD-55918
NRC number1579
NPARC number3538272
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Record identifier133f6735-0f08-4eef-9566-96d1998f7eab
Record created2009-03-01
Record modified2016-05-09
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