Chemical chaperoning action of glycerol on the antifreeze protein of rainbow smelt

Alternative titleChemical chaperone action of glycerol on the antifreeze protein of rainbow smelt (Osmerus mordax)
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DOIResolve DOI: http://doi.org/10.1016/j.jtherbio.2010.11.006
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TypeArticle
Journal titleJournal of Thermal Biology
Volume36
Issue1
Pages7883; # of pages: 6
SubjectGlycerol; Trimethylamine oxide; Chemical chaperone; Proteostasis; Antifreeze protein; Cold adaptation
AbstractRainbow smelt (Osmerus mordax) accumulate high levels of glycerol and moderate levels of trimethylamine oxide (TMAO) that lower the colligative freezing point of the serum and thereby contribute to seasonal freeze resistance. In the current study, the possibility that one or both of these compounds might also have a chaperoning role at low temperatures in smelt was investigated by studying their effects on the smelt antifreeze protein (AFP). Activity of the AFP in the presence of physiological levels of TMAO and glycerol was observed by means of ice crystal morphology and measured as thermal hysteresis. Ice crystals in AFP solutions were not visibly modified by either compound and TMAO at 25 and 50 mM had no appreciable effect on hysteresis; however, glycerol at 250 and 500 mM increased hysteresis. An equiosmolar level of NaCl was not as effective as glycerol in enhancing hysteresis, suggesting that osmolarity had little or no role. Although cross-linking experiments showed dimerization of AFP to be unchanged in the presence of glycerol, circular dichroism and intrinsic fluorescence analyses revealed enhanced protein folding. As glycerol enhances the folding and consequent activity of smelt AFP, protein chaperoning appears to be an endogenous role of glycerol in this vertebrate species.
Publication date
PublisherElsevier
LanguageEnglish
AffiliationNational Research Council Canada; NRC Institute for Marine Biosciences
Peer reviewedYes
NRC number51751
NPARC number21268214
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Record identifier137b6e84-0b1b-4be1-a2ae-f9f69a6f43d9
Record created2013-06-05
Record modified2017-03-23
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