Expression and production of human interleukin-7 in insect cells using baculovirus expression vector system (BEVS)

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DOIResolve DOI: http://doi.org/10.1007/s12010-008-8178-3
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TypeArticle
Journal titleApplied Biochemistry and Biotechnology
Volume151
Issue1
Pages93103; # of pages: 11
Subjectbio; Cells; Human; insect cell; protein; therapy; Interleukin-7; insect cells; Trichoplusia ni cells; glycoprotein cytokine production; baculovirus vector system; batch bioreactor
AbstractInterleukin-7 (IL-7) is a glycoprotein cytokine with significant clinical and biomedical potential, such as cancer therapy and HIV infections. Earlier it has been cloned and expressed in various protein expression systems; however, they are not efficient for large-scale production. To address this inadequacy, we report in this paper the production of recombinant human interleukin-7 (hIL-7) in insect cells. A recombinant bacmid containing hIL-7 was constructed, purified, and characterized. It was used to infect Trichoplusia ni (BT1-TN-5B1/High Fivetrade mark) insect cells. Result shows that T. ni cells successfully produce hIL-7 in shake flask cultures. A scale up to 2.5-L laboratory batch bioreactor showed the efficacy of this system for large-scale production. Our results offer a highly efficient, inexpensive, and convenient system for the large-scale expression and production of recombinant hIL-7.
Publication date
LanguageEnglish
AffiliationNRC Biotechnology Research Institute; National Research Council Canada (NRC-CNRC)
Peer reviewedNo
NRC number47824
NPARC number12400998
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Record identifier14c6ec84-334e-49a7-8a76-2f4ccac6ffb4
Record created2009-10-03
Record modified2016-05-09
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