Analysis of a ferric uptake regulator (Fur) knockout mutant in Aeromonas salmonicida subsp. salmonicida

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DOIResolve DOI: http://doi.org/10.1016/j.vetmic.2012.10.038
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TypeArticle
Journal titleVeterinary Microbiology
ISSN0378-1135
Volume162
Issue2-Apr
Pages831841; # of pages: 11
Subjectferric uptake regulator; iron; outer membrane protein C; siderophore; Aeromonas hydrophila; Aeromonas salmonicida; amino acid sequence; article; autoregulation; bacterial gene; bacterial growth; bacterial strain; Escherichia coli; gene deletion; nonhuman; open reading frame; protein analysis; protein purification; proteomics; reverse transcription polymerase chain reaction; sequence analysis; wild type; Aeromonas hydrophila; Aeromonas salmonicida; Aeromonas salmonicida subsp. salmonicida; Bacteria (microorganisms); Escherichia coli; Salmonidae
AbstractAeromonas salmonicida subsp. salmonicida is the etiological agent of furunculosis; a serious infectious disease in aquaculture raised salmonids. Iron acquisition has been shown to be critical for the survival of pathogenic bacteria during the course of infection. Previous work has demonstrated that A. salmonicida expresses iron-repressible IROMP proteins, suggesting the presence of iron acquisition systems that are under the control of a ferric uptake regulator (Fur). In this study, the A. salmonicida fur has been sequenced and a fur deletion strain generated. The A. salmonicida fur gene has an open reading frame of 428. bp, coding for a protein of 143 amino acids, and with high homology to previously described Fur proteins. The Fur protein product had a 94% sequence identity and 96% sequence similarity to the Aeromonas hydrophila Fur protein product. Transcription of the A. salmonicida fur gene was not regulated by the iron status of the bacterium and is not autoregulated, as in Escherichia coli. Proteomic analysis of the A. salmonicida fur mutant, fails to repress iron-regulated outer membrane proteins in the presence of iron. The A. salmonicida fur::KO mutant shows significantly reduced pathogenicity compared to the wild-type parental strain. In addition, the A. salmonicida fur mutant provides an important tool for further investigation of the iron acquisition mechanisms utilized by A. salmonicida. © 2012 Elsevier B.V..
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); NRC Institute for Marine Biosciences (IMB-IBM)
Peer reviewedYes
NPARC number21269914
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Record identifier156e9412-7ed7-4717-aace-673678c1f363
Record created2013-12-13
Record modified2016-05-09
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