High-level recombinant protein production in bioreactors using the baculovirus-insect cell expression system

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DOIResolve DOI: http://doi.org/10.1002/bit.260361108
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TypeArticle
Journal titleBiotechnology and Bioengineering
Volume36
Issue11
Pages11331140; # of pages: 8
AbstractIn order to develop an efficient process for large-scale production of recombinant protein, various factors were studied which affect the productivity of Sf-9 (Spodoptera frugiperda) insect cells when using the baculovirus expression system. It was shown that upon infection with the Bac-BRV6L recombinant baculovirus, the level per cell of VP6 (a bovine rotavirus nucleocapsid protein) would drop 10-fold when host cell density at the time of infection increased from 2 × 10^6 to 3 × 10^6 cells/mL. The decrease was found to be totally reversible by culture medium renewal after infection, even when cells were infected at the stationary phase. Recombinant protein production was 4–6 times higher using TNMFH medium supplemented with 10% fetal bovine serum (FBS) than in IPL/41 serum-free medium. Fine-tuning of infection parameters in a 4-L surface-aerated bioreactor resulted in the production of typically 350 mg/L of VP6 protein, representing more than 25% of total cell proteins.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada; NRC Biotechnology Research Institute
Peer reviewedNo
NPARC number12327863
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Record identifier158ed188-4458-4d54-bfb1-63abb3249437
Record created2009-09-10
Record modified2016-05-09
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