Complete nucleotide sequence and mRNA-mapping of the large subunit gene of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) from Chlamydomonas moewusii

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DOIResolve DOI: http://doi.org/10.1016/0378-1119(86)90330-6
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TypeArticle
Journal titleGene
Volume50
Issue1-3
Pages259270; # of pages: 12
Subjectrecombinant DNA; amino acid sequence; transcriptional organization; green alga; mung bean nuclease; transcript mapping
AbstractNucleotide (nt) sequence of the large subunit (LS) gene of ribulose-1,5-bisphosphate carboxylase/oxygenase from the green alga, Chlamydomonas moewusii, and mapping of transcription ends was achieved by two new strategies. The deduced LS sequence of 475 amino acid residues was compared with similar genes from six others species; cyanobacteria, land plants and a related alga (C. reinhardtii). The most conserved regions are the three ribulose bisphosphate binding sites and the CO2 activator site. The nt sequence conservation outside the coding region is limited to only three segments within the 5′-flanking region: a region of tandem repeats, TATAA box and ribosome-binding site. Termination point of transcription is an ‘A’ residue 3′ to the first of two 18-nt inverted repeats, which has the potential to form a stem-loop hairpin structure. The possible role of these potential regulatory features for transcription and translation, and similar structures in other LS genes is presented.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada; NRC Institute for Biological Sciences
Peer reviewedYes
NRC numberYANG1986
NPARC number9384300
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Record identifier170ad881-7a7c-4391-bf23-3af27ce21f94
Record created2009-07-10
Record modified2016-05-09
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