Dynamics of lipid droplets induced by the hepatitis C virus core protein

Download
  1. Get@NRC: Dynamics of lipid droplets induced by the hepatitis C virus core protein (Opens in a new window)
DOIResolve DOI: http://doi.org/10.1016/j.bbrc.2010.07.101
AuthorSearch for: ; Search for: ; Search for: ; Search for: ; Search for:
TypeArticle
Journal titleBiochemical and Biophysical Research Communications
Volume399
Pages518524; # of pages: 7
Subjectdynamics of lipid droplets; hepatitis C virus; protein; CARS microscopy; Particle tracking; Dynamics; Microtubules
AbstractThe hepatitis C virus (HCV) is a global health problem, with limited treatment options and no vaccine available. HCV uses components of the host cell to proliferate, including lipid droplets (LD) onto which HCV core proteins bind and facilitate viral particle assembly. We have measured the dynamics of HCV core protein-mediated changes in LDs and rates of LD movement on microtubules using a combination of coherent anti-Stokes Raman scattering (CARS), two-photon fluorescence (TPF), and differential interference contrast (DIC) microscopies. Results show that the HCV core protein induces rapid increases in LD size. Particle tracking experiments show that HCV core protein slowly affects LD localization by controlling the directionality of LD movement on microtubules. These dynamic processes ultimately aid HCV in propagating and the molecules and interactions involved represent novel targets for potential therapeutic intervention.
Publication date
LanguageEnglish
AffiliationNRC Steacie Institute for Molecular Sciences; National Research Council Canada
Peer reviewedYes
NRC publication
This is a non-NRC publication

"Non-NRC publications" are publications authored by NRC employees prior to their employment by NRC.

NPARC number16925469
Export citationExport as RIS
Report a correctionReport a correction
Record identifier198291d4-c59e-4f35-b6d7-cd387ba49765
Record created2011-02-25
Record modified2016-05-09
Bookmark and share
  • Share this page with Facebook (Opens in a new window)
  • Share this page with Twitter (Opens in a new window)
  • Share this page with Google+ (Opens in a new window)
  • Share this page with Delicious (Opens in a new window)