The engineering of bacteria bearing azido-pseudaminic acid modified flagella

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DOIResolve DOI: http://doi.org/10.1002/cbic.200900018
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TypeArticle
Journal titleChembioChem
Volume10
Issue8
Pages13171320; # of pages: 4
Subjectazides; C. jejuni; cell surfaces; flagella; metabolism; Staudinger reaction
AbstractWe have demonstrated that by feeding nonmotile mutant C. jejuni bacteria with a neutral azide-labelled pseudaminic acid precursor we can restore their ability to generate functional flagella. The presence of azido-pseudaminic acid on the surface of the flagella provides a bio-orthogonal chemical handle that can be used to modify the flagellar proteins.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); NRC Institute for Biological Sciences
Peer reviewedYes
NPARC number15295298
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Record identifier1a6e4b23-6334-4fbc-b396-b58ebd9a0efa
Record created2010-05-17
Record modified2016-05-09
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