Studies on lipid peroxidation in normal and tumour tissues. The Novikoff rat liver tumour

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DOIResolve DOI: http://doi.org/10.1042/bj2350507
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TypeArticle
Journal titleBiochemical Journal
ISSN0264-6021
Volume235
Issue2
Pages507514; # of pages: 8
Subjectalpha tocopherol; cytochrome p450; lipid peroxide; reduced nicotinamide adenine dinucleotide phosphate ferrihemoprotein reductase; cell culture; in vitro study; liver; nonhuman; novikoff hepatoma; priority journal; rat; Animals; Cholesterol; Cytochrome P-450 Enzyme System; Fatty Acids; Gamma Rays; Lipid Metabolism; Liver; Liver Neoplasms, Experimental; Male; Microsomes, Liver; NADPH-Ferrihemoprotein Reductase; Oxidation-Reduction; Proteins; Rabbits; Rats; Rats, Inbred Strains; Vitamin E
AbstractA study has been made of the factors that contribute to the decreased rates of lipid peroxidation under different pro-oxidant conditions in intact Novikoff tumour cells, and in microsomal suspensions prepared from Novikoff tumour cells, compared with isolated normal rat hepatocytes and microsomal suspensions prepared from normal rat liver. The pro-oxidant conditions were the addition of either NADPH, NADPH + ADP + iron, NADPH + CCl4 or ascorbate + iron to the experimental systems used, or exposure to γ-radiation. Contributory factors to the lower rates of lipid peroxidation observed include: (a) a significant decrease in the polyunsaturated fatty acid content of Novikoff cells or Novikoff microsomes; the decreases are especially marked for the C(20:4) and C(22:6) fatty acids; (b) a very marked reduction in NADPH-cytochrome c reductase; and (c) no detectable content of cytochrome P-450. Another, and in our opinion critical, contribution to the diminished rate of lipid peroxidation in the tumour material is the substantial increase in α-tocopherol relative both to total lipid and to methylene-interrupted double bonds in fatty acids. Moreover, the α-tocopherol is the major contributor to lipid-soluble chain-breaking antioxidant in lipid extracts of normal liver and of Novikoff tumour material.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC)
Peer reviewedYes
NPARC number21276741
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Record identifier1b04f342-e6ea-4864-84a5-11d08add168a
Record created2015-10-13
Record modified2016-05-09
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