Carbon dioxide in tissues, cells, and biological fluids detected by FTIR spectroscopy

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DOIResolve DOI: http://doi.org/10.1021/jp953254t
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TypeArticle
Journal titleThe Journal of Physical Chemistry
Volume100
Issue16
Pages68456848; # of pages: 4
AbstractPrevious studies from this lab have demonstrated the presence of a novel absorption in infrared spectra of synovial fluid films (2337−2343 cm-1) which was suggested to arise from CO2 trapped within the organic matrix left after drying. In the present paper, we establish the presence of this absorption in a wide range of biological fluids, tissues, and cell suspensions. Results of studies with a range of common biological materials suggest that the CO2 interacts nonspecifically with the protein components of cells and fluids. Experiments with 13C-labeled glucose clearly demonstrate that this ubiquitous absorption is attributable to CO2 produced by glucose breakdown within cells, presenting the interesting possibility of monitoring cellular metabolism by infrared spectroscopy.
Publication date
PublisherAmerican Chemical Society
LanguageEnglish
AffiliationNational Research Council Canada; NRC Institute for Biodiagnostics
Peer reviewedYes
NRC number405
NPARC number9741984
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Record identifier1b336e0d-dc94-473f-9359-110f9fd44bf4
Record created2009-07-17
Record modified2017-03-23
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