Application of double-spike isotope dilution for the accurate determination of Cr(III), Cr(VI) and total Cr in yeast

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DOIResolve DOI: http://doi.org/10.1007/s00216-006-0756-z
AuthorSearch for: ; Search for: ; Search for: ; Search for:
TypeArticle
Journal titleAnalytical and Bioanalytical Chemistry
ISSN1618-2642
Volume386
IssueNovember 6
Pages16731680; # of pages: 8
SubjectCr(III); Cr(VI); Double-spike isotope dilution; Yeast; ICP-MS
AbstractA method is presented for the simultaneous determination of Cr(III) and Cr(VI) in yeast using species-specific double-spike isotope dilution (SSDSID) with anion-exchange liquid chromatography (LC) separation and sector field inductively coupled plasma mass spectrometric (SF-ICP-MS) detection. Total Cr is quantitated using ID SF-ICP-MS. Samples were digested on a hot plate at 95±2 °C for 6 h in an alkaline solution of 0.5 M NaOH and 0.28 M Na2CO3 for the determination of Cr(III) and Cr(VI), whereas microwave-assisted decomposition with HNO3 and H2O2 was used for the determination of total Cr. Concentrations of 2,014±16, 1,952±103 and 76±48 mg kg−1 (one standard deviation, n=4, 3, 3), respectively were obtained for total Cr, Cr(III) and Cr(VI) in the yeast sample. Significant oxidation of Cr(III) to Cr(VI) (24.2±7.6% Cr(III) oxidized, n=3) and reduction of Cr(VI) to Cr(III) (37.6±6.5% Cr(VI) reduced, n=3 ) occurred during alkaline extraction and subsequent chromatographic separation at pH 7. Despite this significant bidirectional redox transformation, quantitative recoveries for both Cr(III) and Cr(VI) were achieved using the SSDSID method. In addition, mass balance between total Cr and the sum of Cr(III) and Cr(VI) concentrations was achieved. Method detection limits of 0.3, 2 and 30 mg kg−1 were obtained for total Cr, Cr(VI) and Cr(III), respectively, based on a 0.2-g sub-sample.
Publication date
LanguageEnglish
AffiliationNRC Institute for National Measurement Standards; National Research Council Canada
Peer reviewedNo
Identifier19834561
NRC number3993
NPARC number8900274
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Record identifier1be167a4-3374-4244-a25a-0deab054e8b1
Record created2009-04-22
Record modified2016-05-09
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