Metabolite production during transformation of 2,4,6-TNT by a mixed culture acclimated and maintained on crude oil-containing media

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DOIResolve DOI: http://doi.org/10.1007/s00253-004-1625-3
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TypeArticle
Journal titleApplied Microbiology and Biotechnology
Volume65
Issue6
Pages739746; # of pages: 8
Subjectenv
AbstractMetabolites formed during 2,4,6-trinitrotoluene (TNT) removal by a mixed bacterial culture ( acclimated and maintained on crude oil-containing medium and capable of high rates of TNT removal) were characterized. In resting cell experiments in the absence of glucose, 46.2 mg/l TNT were removed in 171 h (87.5% removal), with a combined total formation of 7.7 mg/l amino-4,6-dinitrotoluene (ADNT) and 0.3 mg/l 4,4'-azoxytetranitrotoluene and 2',4-azoxytetranitrotoluene, leaving 70% of the initial TNT unaccounted for. In the presence of glucose, resting cells removed 45.4 mg/l TNT in 49 h (95.5% removal), with 9.1 mg/l ADNT and 2.4 mg/l azoxy compounds being produced, leaving 70.3% of the TNT unaccounted for. Growing cells ( glucose present) were capable of removing 44.2 mg/l TNT within 21 h (97.9% removal), with the concomitant formation of 1.8 mg/l ADNTs and 2.2 mg/l azoxy compounds. Denitrated TNT in the form of 2,6-dinitrotoluene was also produced in growing cells with a maximum amount of 1.31 mg/l after 28 h, followed by a slight decrease with time, leaving 88.5% of the initial TNT unaccounted for after 171 h. Radiolabeled 14C-TNT studies revealed 4.14% mineralization after an incubation period of 163 days with growing cells.
Publication date
LanguageEnglish
AffiliationNRC Biotechnology Research Institute; National Research Council Canada
Peer reviewedNo
NRC number45967
NPARC number3539130
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Record identifier1d6dbc5a-8b93-4122-ba07-140257fdddea
Record created2009-03-01
Record modified2016-05-09
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