Profiling of cytokine and chemokine responses using multiplex bead array technology

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DOIResolve DOI: http://doi.org/10.1007/978-1-62703-589-7_16
AuthorSearch for: ; Search for:
TypeArticle
Journal titleMethods in Molecular Biology
ISSN1064-3745
ISBN9781627035880
Volume1061
Pages265278; # of pages: 14
Subjectchemokine; CXCL3 chemokine; cytokine; interleukin 1beta; interleukin 6; keratinocyte derived chemokine; macrophage inflammatory protein 1beta; monocyte chemotactic protein 1; RANTES; tumor necrosis factor alpha; animal tissue; article; blood analysis; controlled study; immunology test kit; lung lavage; microarray analysis; mouse; multiplex bead array assay; nonhuman; priority journal; quantitative analysis; supernatant; technology; tissue culture; tissue homogenate
AbstractMultiplex bead array technology expands upon the principles of the enzyme-linked immunosorbent assay by allowing the simultaneous quantification of a large number of cytokines and chemokines within a single sample. This allows for the researcher more freedom to investigate complex immune responses both in vivo and in vitro. Here we describe the detailed assay protocol and technical tips for simultaneous quantification of multiple cytokines and chemokines in mouse biological fluids such as sera, bronchoalveolar lavage fluid, tissue homogenate supernatant, and tissue culture supernatant, using a multiplex bead array assay. © 2013 Springer Science+Business Media, LLC.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); Human Health Therapeutics (HHT-TSH)
Peer reviewedYes
NPARC number21269941
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Record identifier1dd8edd8-90ee-4d6b-a704-1cdf800d7e3a
Record created2013-12-13
Record modified2016-05-09
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