Raman-based detection of bacteria using silver nanoparticles conjugated with antibodies

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DOIResolve DOI: http://doi.org/10.1039/b701160a
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TypeArticle
Journal titleThe Analyst
Volume132
Issue7
Pages679686; # of pages: 8
AbstractSurface enhanced Raman scattering (SERS) has been used to detect bacteria captured by polyclonal antibodies sorbed onto protein-A-modified silver nanoparticles. The selectivity and discrimination of the technique were assured by using a specific antibody to the model bacterium, Escherichia coli. As the SERS enhancement mechanism depends upon the metal surface proximity, 8 nm was considered as the optimum distance between the bacterium and the nanoparticle surface. Spectral reproducibility was verified using Principal Components Analysis to differentiate the clusters corresponding to the biomolecules and/or bacteria sorbed onto nanoparticles. Compared to the normal Raman spectrum, the SERS technique resulted in an intensity enhancement of over 20-fold. Graphical abstract image for this article
Publication date
LanguageEnglish
AffiliationNRC Biotechnology Research Institute; National Research Council Canada
Peer reviewedNo
NRC number49055
NPARC number3539012
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Record identifier1e039504-3b16-4cc3-99c0-1535bba6a1d9
Record created2009-03-01
Record modified2016-05-09
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