Fourier resolution enhancement of infrared spectral data

Download
  1. Get@NRC: Fourier resolution enhancement of infrared spectral data (Opens in a new window)
DOIResolve DOI: http://doi.org/10.1016/0076-6879(92)10011-2
AuthorSearch for: ; Search for:
TypeBook Chapter
Book titleNumerical Computer Methods
Series titleMethods in Enzymology; Volume 210
ISSN00766879
ISBN9780121821111
Pages192200
AbstractThis chapter presents a critical discussion of the computational methods of Fourier resolution enhancement (band narrowing) and their role in the analysis of the infrared absorption spectra of proteins. This type of numerical data processing does not affect the instrumental resolution but changes the shape of the infrared bands and, by reducing their width, allows a better visual separation (that is, resolution) of individual component bands. One area of biology in which infrared spectroscopy has become particularly useful is the structural analysis of proteins and, in particular, the determination of the secondary structure of soluble and membrane-bound proteins. There are two common methods of resolution enhancement via band narrowing that use the Fourier transform: Fourier self-deconvolution (FSD) and Fourier derivation (FD). The chapter discusses a number of practical considerations in the application of the numerical computer methods for band narrowing. The Fourier resolution enhancement in applications may be employed to advantage on any data set that satisfies two criteria: (1) high S/N ratio and (2) composite bands with intrinsic widths that are large as compared to the instrumental resolution.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada; NRC Steacie Institute for Molecular Sciences
Peer reviewedYes
NRC number325
NPARC number9742263
Export citationExport as RIS
Report a correctionReport a correction
Record identifier1e8c00f0-4ca0-42d8-adfc-c9c408da0c7f
Record created2009-07-17
Record modified2017-01-13
Bookmark and share
  • Share this page with Facebook (Opens in a new window)
  • Share this page with Twitter (Opens in a new window)
  • Share this page with Google+ (Opens in a new window)
  • Share this page with Delicious (Opens in a new window)
Date modified: