Aeromonas hydrophila flagella glycosylation: Involvement of a lipid carrier

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Journal titlePLoS ONE
Article numbere89630
Subjectferredoxin nitrite reductase; flagellin; lipopolysaccharide; Aeromonas hydrophila; bacterial strain; bacterium isolation; biosynthesis; cell motility; controlled study; DNA fragmentation; enzyme specificity; epimerization; glycosylation; microscopy; molecular model; molecular weight; nucleotide sequence; plasmid; polymerase chain reaction; protein analysis; protein modification; protein purification; sequence alignment; tandem mass spectrometry; ubiquitination; Acetylgalactosamine; Amino Acid Sequence; Carbohydrate Conformation; Carbohydrate Sequence; Carrier Proteins; Gene Expression; Lipid Metabolism; Molecular Sequence Data; Protein Processing, Post-Translational
AbstractPolar flagellin proteins from Aeromonas hydrophila strain AH-3 (serotype O34) were found to be O-glycosylated with a heterogeneous glycan. Mutants unable to produce WecP or Gne enzymes showed altered motility, and the study of their polar flagellin glycosylation showed that the patterns of glycosylation differed from that observed with wild type polar flagellin. This suggested the involvement of a lipid carrier in glycosylation. A gene coding for an enzyme linking sugar to a lipid carrier was identified in strain AH-3 (WecX) and subsequent mutation abolished completely motility, flagella production by EM, and flagellin glycosylation. This is the first report of a lipid carrier involved in flagella O-glycosylation. A molecular model has been proposed. The results obtained suggested that the N -acetylhexosamines are N-acetylgalactosamines and that the heptasaccharide is completely independent of the O34-antigen lipopolysaccharide. Furthermore, by comparing the mutants with differing degrees of polar flagellin glycosylation, we established their importance in A. hydrophila flagella formation and motility.
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AffiliationNational Research Council Canada; Human Health Therapeutics
Peer reviewedYes
NPARC number21272940
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Record identifier1e9c156f-d85b-47f0-a1e7-a50503ab5b56
Record created2014-12-03
Record modified2016-05-09
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