Structural analysis of the phase-variable lipooligosaccharide from Haemophilus somnus strain 738

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TypeArticle
Journal titleEuropean Journal of Biochemistry
Volume253
Issue2
Pages507516; # of pages: 10
SubjectACID; analysis; Canada; chemistry; COMPONENT; COMPONENTS; CONFIGURATION; ELUCIDATION; Glucose; Haemophilus; isolation & purification; KDO; lipooligosaccharide; LIPOPOLYSACCHARIDE; Lipopolysaccharides; Magnetic Resonance Spectroscopy; mass spectrometry; MASS-SPECTROMETRY; Methylation; NMR spectroscopy; oligosaccharide; Oligosaccharides; PHASE; POTENTIAL; Spectrum Analysis,Mass; STRAIN; structural analysis; structure; UNIT
AbstractThe structure of the phase variable lipooligosaccharide (LOS) from Haemophilus somnus strain 738 was elucidated. The LOS was subjected to a variety of degradative procedures. The structures of the purified products were established by monosaccharide and methylation analyses, NMR spectroscopy and mass spectrometry. The following structures for the two major components were determined on the basis of the combined data from these experiments. [structure in text]. In the structures Kdo is 3-deoxy-D-manno-octulosonic acid, PEtn is phosphoethanolamine, PCho is phosphocholine, Hep is L-glycero-D-manno-heptose, and the remaining glucose units have the D configuration. The elucidation of these structures has increased our understanding of the relationship between the phase-variable LOS and the pathogenic potential of this organism
Publication date
LanguageEnglish
AffiliationNRC Institute for Biological Sciences; National Research Council Canada
Peer reviewedYes
NRC numberCOX1998
NPARC number9360475
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Record identifier1f1a5426-2062-43d9-b393-f6e60c1ffa62
Record created2009-07-10
Record modified2016-06-01
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