Analysis of B-genome chromosome introgression in interspecific hybrids of Brassica napus x B. carinata

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DOIResolve DOI: http://doi.org/10.1534/genetics.110.124925
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TypeArticle
Journal titleGenetics
ISSN0016-6731
Volume187
Issue3
Pages659673; # of pages: 15
Subjectagronomic trait; article; Brassica carinata; chromosome analysis; chromosome pairing; chromosome rearrangement; controlled study; gene mapping; genetic linkage; genetic marker; genetic recombination; genome analysis; genomic in situ hybridization; inheritance; introgression; morphological trait; nonhuman; plant chromosome; plant genetics; plant genome; priority journal; rapeseed; root vegetable; tetraploidy; Brassica; Brassica napus; Chimera; Genetic Linkage; Genome, Plant; Hybridization, Genetic; In Situ Hybridization, Fluorescence; Minisatellite Repeats; Polyploidy; Brassica; Brassica carinata; Brassica napus
AbstractBrassica carinata, an allotetraploid with B and C genomes, has a number of traits that would be valuable to introgress into B. napus. Interspecific hybrids were created between B. carinata (BBCC) and B. napus (AACC), using an advanced backcross approach to identify and introgress traits of agronomic interest from the B. carinata genome and to study the genetic changes that occur during the introgression process. We mapped the B and C genomes of B. carinata with SSR markers and observed their introgression into B. napus through a number of backcross generations, focusing on a BC3 and BC3S 1 sibling family. There was close colinearity between the C genomes of B. carinata and B. napus and we provide evidence that B. carinata C chromosomes pair and recombine normally with those of B. napus, suggesting that similar to other Brassica allotetraploids no major chromosomal rearrangements have taken place since the formation of B. carinata. There was no evidence of introgression of the B chromosomes into the A or C chromosomes of B. napus; instead they were inherited as whole linkage groups with the occasional loss of terminal segments and several of the B-genome chromosomes were retained across generations. Several BC3S1 families were analyzed using SSR markers, genomic in situ hybridization (GISH) assays, and chromosome counts to study the inheritance of the B-genome chromosome(s) and their association with morphological traits. Our work provides an analysis of the behavior of chromosomes in an interspecific cross and reinforces the challenges of introgressing novel traits into crop plants. Copyright © 2011 by the Genetics Society of America.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); NRC Plant Biotechnology Institute (PBI-IBP)
Peer reviewedYes
NPARC number21271381
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Record identifier1f1fae04-b542-413f-b587-41283463c3ad
Record created2014-03-24
Record modified2016-05-09
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