Real-time cell-impedance sensing assay as an alternative to clonogenic assay in evaluating cancer radiotherapy

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DOIResolve DOI: http://doi.org/10.1007/s00216-011-4934-2
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TypeArticle
Journal titleAnalytical and Bioanalytical Chemistry
ISSN1618-2642
Volume400
Issue7
Pages20032011; # of pages: 9
SubjectCancer radiotherapy; Cell radiosensitivity; Clonogenic assays; Gold nanoparticle; RT-CES cell-proliferation assay; Radiotherapy; cell survival; neoplasm; pathology; radiation dose; treatment outcome; tumor cell line; Neoplasms; Radiation Tolerance
AbstractIntrinsic radiosensitivity of normal and tumour tissues has been shown to be an independent prognostic factor for patients' response to radiotherapy. This study compares the real-time cell-impedance sensing (RT-CES) assay with the conventional clonogenic assay in terms of in-vitro radiosensitivity. One objective in this study was to predict in-vivo response to gold nanoparticle (GNP) treatment on the basis of in-vitro RT-CES testing results. Four adenocarcinoma cancer cell lines were tested using both the RT-CES and the clonogenic assays. Cell-survival curves were plotted, and the mean SF2 values obtained by these two different assay methods were compared using ANOVA. Radiation sensitivities obtained in-vitro were then compared with the in-vivo results. On the basis of the measurement of cell colonies, the RT-CES assay has similar radiosensitivity to the clonogenic assay, but significantly shortens the testing time from 14-21 days to only 72 h. Intrinsic GNP enhanced radiation sensitivity using tumour volume (mm3) in vivo is comparable with that using RT-CES cell survival assay in vitro. Furthermore, the RT-CES system provides real-time information regarding the state of cell radiosensitivity that may give useful information towards personalizing radiotherapy. The RT-CES assay enables more reliable and time-efficient results in the evaluation of radiosensitivity. [Figure not available: see fulltext.] © 2011 Springer-Verlag.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); National Institute for Nanotechnology
Peer reviewedYes
NPARC number21272017
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Record identifier2172f83c-3e06-434c-967a-4d609f058440
Record created2014-05-21
Record modified2016-05-09
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