Separation of resin acids using cyclodextrin modified capillary electrophoresis

Download
  1. Get@NRC: Separation of resin acids using cyclodextrin modified capillary electrophoresis (Opens in a new window)
AuthorSearch for: ; Search for: ; Search for: ; Search for:
TypeArticle
Journal titleElectrophoresis
Volume20
Pages15461554; # of pages: 9
SubjectResin acids; Capillary electrophoresis; Methyl-β-cyclodextrin; Sulfobutylether-β-cyclodextrin
AbstractA cyclodextrin-modified capillary electrophoretic method has been developed for the analysis of eleven common resin acids using a pH 4.5, 20 mM sodium acetate buffer containing 10% acetonitrile, 20 mM methyl-β-cyclodextrin (MECD) and 30 mM sulfobutylether-β-cyclodextrin (SBCD) as buffer modifiers. At pH below their pKa (< 5.7—6.4) the resin acids were virtually unionized and insoluble; however, they formed water-soluble inclusion complexes with MECD (20 mM) or SBCD (30 mM) even at pH 4.5. The analytes were separated in 25 min and, with the exception of two pairs, 12- or 14-chlorodehydroabietic/12,14-dichlorodehydroabietic acid and dehydroabietic/palustric acid, the remaining resin acids were baseline-separated. Analysis time was significantly shortened (< 12 min) at pH 9.25 using 30 mM SBCD and 20 mM MECD in 20 mM sodium borate. Resin acids were baseline-separated with the exception of two pairs, pimaric/ sandaracopimaric acid and 12- or 14-chlorodehydroabietic/abietic acid. The addition of 7.5% methanol to the running buffer resolved the abietic acid peak. Both HPLC and micellar capillary electrokinetic chromatography using 20 mM deoxycholic acid, 10% acetonitrile in 20 mM sodium borate, pH 9.25, failed to resolve the resin acids. The simple capillary electrophoretic method developed would be useful for the rapid separation and characterization of several important resin acids in pulp mill effluents and other contaminated samples.
Publication date
LanguageEnglish
AffiliationNRC Biotechnology Research Institute; National Research Council Canada
Peer reviewedNo
NRC number41847
NPARC number3539618
Export citationExport as RIS
Report a correctionReport a correction
Record identifier2247dbad-09cb-466f-b285-6b35a0a5a144
Record created2009-03-01
Record modified2016-05-09
Bookmark and share
  • Share this page with Facebook (Opens in a new window)
  • Share this page with Twitter (Opens in a new window)
  • Share this page with Google+ (Opens in a new window)
  • Share this page with Delicious (Opens in a new window)