Efficient and rapid uptake of magnetic carbon nanotubes into human monocytic cells: implications for cell-based cancer gene therapy

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DOIResolve DOI: http://doi.org/10.1007/s10529-012-0858-y
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TypeArticle
Journal titleBiotechnology Letters
ISSN0141-5492
Volume34
Issue5
Pages989993; # of pages: 5
SubjectCancer; Cellular and nuclear uptake; Fluorescein isothiocyanate; Gene therapies; Magnetic carbon nanotubes; Monocytic leukemia cells
AbstractMonocyte-based gene therapies in cancer have been hampered by either the resistance of these cells to non-viral molecular delivery methods or their poor trafficking to the tumor site after their ex vivo manipulations. Magnetic nanoparticles (MNP)-loaded genetically engineered monocytes can efficiently delivered to tumor site by external magnetic field, but they are not ideal delivery tools due to their spherical shape. Hence, we have investigated the cellular uptake efficiency and cytotoxicity of fluorescein isothiocyanate (FITC)-labelled magnetic carbon nanotubes (FITC-mCNT) in human monocytic leukemia cell line THP-1 for application in cell-based gene therapy against cancer. Uptake of FITC-mCNT into THP-1 cells reached 100% only 1 h after the delivery. Confocal imaging confirmed that FITC-mCNT entered the cell cytoplasm and even into the nucleus. FITC-mCNT uptake did not compromise cell viability. This delivery system might therefore enhance cell-based cancer gene therapies.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); Security and Disruptive Technologies
Peer reviewedYes
NPARC number21270217
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Record identifier2265506a-9011-4482-89f5-83a5e75598be
Record created2014-01-13
Record modified2016-05-09
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