Rapid fabrication and chemical patterning of polymer microstructures and their applications as a platform for cell cultures

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DOIResolve DOI: http://doi.org/10.1007/s10544-005-3023-8
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TypeArticle
Journal titleBiomedical Microdevices
ISSN1387-2176
Volume7
Issue3
Pages179184; # of pages: 6
Subjectrapid fabrication method; chemical patterning; surface modification techniques; microstructured substrates; guided cell culture
AbstractMuch of the current knowledge regarding biological processes has been obtained through in-vitro studies in bulk aqueous solutions or in conventional Petri-dishes, with neither methodology accurately duplicating the actual in-vivo biological processes. Recently, a number of innovative approaches have attempted to address these shortcomings by providing substrates with controlled features. In particular, tunable surface chemistries and topographical micro and nanostructures have been used as model systems to study the complex biological processes.We herein report a versatile and rapid fabrication method to produce a variety of microstructured polymer substrates with precise control and tailoring of their surface chemistries. A poly(dimethylsiloxane) (PDMS) substrate, produced by replication over a master mold with specific microstructures, is modified by a fluoro siloxane derivative to enhance its anti-adhesion characteristics and used as a secondary replication mold. A curable material, deposited by spin coating on various substrates, is stamped with the secondary mold and crosslinked. The removal of the secondary mold produces a microstructured surface with the same topographical features as the initial master mold. The facile chemical patterning of the microstructured substrates is demonstrated through the use of microcontact printing methods and these materials are tested as a platform to guide cell attachment, growth and proliferation. The master mold and flexible fluorinated PDMS stamps can be used in a repeated manner without any degradation of the anti-adhesion characteristics opening thewayto the development of high-throughput fabrication methods that can yield reliable and inexpensive microstructured and chemically patterned substrates.
Publication date
PublisherSpringer
LanguageEnglish
AffiliationNRC Institute for Microstructural Sciences; NRC Institute for Biological Sciences; National Research Council Canada
Peer reviewedYes
NPARC number21264663
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Record identifier23bb5cf9-6cea-4bb0-808c-6e586061ed62
Record created2013-03-20
Record modified2016-05-09
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