High-quality reference genes for quantifying the transcriptional responses of Oryza sativa L. (ssp. indica and japonica) to abiotic stress conditions

Download
  1. (PDF, 1009 KB)
  2. Get@NRC: High-quality reference genes for quantifying the transcriptional responses of Oryza sativa L. (ssp. indica and japonica) to abiotic stress conditions (Opens in a new window)
DOIResolve DOI: http://doi.org/10.1007/s11434-013-5726-1
AuthorSearch for: ; Search for: ; Search for:
TypeArticle
Journal titleChinese Science Bulletin
ISSN1001-6538
1861-9541
Volume58
Issue16
Pages19191930; # of pages: 12
SubjectKDML105; microarrays; quantitative real-time PCR; reference gene; rice (Oryza sativa L.); stress responsive gene
AbstractRice (Oryza sativa L.) is important to food security and is also an excellent model plant for numerous cereal crops. A functional genomics study in rice includes characterization of the expression dynamics of genes by quantitative real-time PCR (qPCR) analysis; this is a significant key for developing rice varieties that perform well in the face of adverse climate change. The qPCR analysis requires the use of appropriate reference genes in order to make any quantitative interpretations meaningful. Here, the new potential reference genes were selected from a huge public database of rice microarray experiments. The expression stability of 14 candidates and 4 conventional reference genes was validated by geNorm PLUS and NormFinder software. Seven candidates are superior to the conventionally used reference genes in qPCR and three genes can be used reliably for quantitating the expression of genes involved in abiotic stress responses. These high-quality references EP (LOC_Os05g08980), HNR (LOC_Os01g71770), and TBC (LOC_Os09g34040) worked very well in three indica genotypes and one japonica genotype. One of indica genotypes including the Jasmine rice, KDML105 developed in Thailand for which no reference genes have been reported until now.
Publication date
PublisherSpringer-Verlag
LanguageEnglish
AffiliationNational Research Council Canada; Aquatic and Crop Resource Development
Peer reviewedYes
NRC number54673
NPARC number21268503
Export citationExport as RIS
Report a correctionReport a correction
Record identifier24c47b36-e930-440b-aaa5-7cc02f193498
Record created2013-09-03
Record modified2016-05-09
Bookmark and share
  • Share this page with Facebook (Opens in a new window)
  • Share this page with Twitter (Opens in a new window)
  • Share this page with Google+ (Opens in a new window)
  • Share this page with Delicious (Opens in a new window)